This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAuroraA-GFP-AURKA-mCherry
catalog :
99878
citations: 2
Reference
Xue J, Zhao Y, Aronowitz J, Mai T, Vides A, Qeriqi B, et al. Rapid non-uniform adaptation to conformation-specific KRAS(G12C) inhibition. Nature. 2020;577:421-425 pubmed publisher
Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M. A FRET biosensor reveals spatiotemporal activation and functions of aurora kinase A in living cells. Nat Commun. 2016;7:12674 pubmed publisher
product information
Catalog Number :
99878
Product Name :
pAuroraA-GFP-AURKA-mCherry
article :
doi10.1038/ncomms12674
id28190005
pubmed_id27624869
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutationCMV promoter was substituted by the AURKA minimal promoter sequence (CTTCCGG) into the AseI/BspEI restriction sites
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression of GFP-AURKA-mCherry in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoterAURKA minimal
sequencing_primer_3
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7
geneAURKA
id6790
genbank_ids
mutation
nameAURKA
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP
locationC terminal on backbone
tagmCherry
resistance markers :
3540
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA