YIp211-P30-His-pol30(K127/164R)-3' | Addgene 99549 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

YIp211-P30-His-pol30(K127/164R)-3'

catalog :

99549

citations: 1

Reference
Davies A, Ulrich H. Detection of PCNA modifications in Saccharomyces cerevisiae. Methods Mol Biol. 2012;920:543-67 pubmed publisher

product information

Catalog Number :

99549

Product Name :

YIp211-P30-His-pol30(K127/164R)-3'

article :

doi	10.1007/978-1-61779-998-3_36
id	28189944
pubmed_id	22941627

bacterial resistance :

Ampicillin

cloning :

backbone

YIplac211

backbone_mutation

backbone_origin

Gietz & Sugino (1988) Gene 74:527-34

backbone_size

3797

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

The ORF is followed by 1129 bp of the POL30 3' region; digest with StuI for integration into URA3 or with PmlI for integration upstream of endogenous POL30 (for exchange of alleles by 5FOA selection.) Please see secondary publication PMID: 19107412 for more information about this plasmid.

growth strain :

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

origin :

pi :

alt_names

PCNA

cloning

clone_method	Restriction Enzyme
cloning_site_3	SphI
cloning_site_5	EcoRI
promoter	POL30
sequencing_primer_3	M13rev
sequencing_primer_5	M13fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YBR088C
gene	POL30
id	852385

genbank_ids

mutation

K127R, K164R

name

POL30

shRNA_sequence

size

2502

species

4932

Saccharomyces cerevisiae