This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YIp128-P30-His-POL30
catalog :
99542
citations: 1
Reference
Davies A, Ulrich H. Detection of PCNA modifications in Saccharomyces cerevisiae. Methods Mol Biol. 2012;920:543-67 pubmed publisher
product information
Catalog Number :
99542
Product Name :
YIp128-P30-His-POL30
article :
doi10.1007/978-1-61779-998-3_36
id28189944
pubmed_id22941627
bacterial resistance :
Ampicillin
cloning :
backboneYIplac128
backbone_mutation
backbone_originGietz & Sugino (1988) Gene 74:527-34
backbone_size4293
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
The ORF is followed by the ADH1 transcription terminator; digest with AflII, BbsI or XcmI for integration into LEU2. Please see secondary publication PMID: 19107412 for more information about this plasmid.
growth strain :
Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker
origin :
37
pi :
alt_names
PCNA
cloning
clone_methodRestriction Enzyme
cloning_site_3SphI
cloning_site_5EcoRI
promoterPOL30
sequencing_primer_3M13rev
sequencing_primer_5M13fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYBR088C
genePOL30
id852385
genbank_ids
mutation
namePOL30
shRNA_sequence
size1569
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tag6xHis
resistance markers :
3522
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA