This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YEp112-CUP1-His-Smt3
catalog :
99537
citations: 1
Reference
Ulrich H, Davies A. In vivo detection and characterization of sumoylation targets in Saccharomyces cerevisiae. Methods Mol Biol. 2009;497:81-103 pubmed publisher
product information
Catalog Number :
99537
Product Name :
YEp112-CUP1-His-Smt3
article :
doi10.1007/978-1-59745-566-4_6
id28189943
pubmed_id19107412
bacterial resistance :
Ampicillin
cloning :
backboneYEplac112
backbone_mutation
backbone_originGietz & Sugino (1988) Gene 74:527-34
backbone_size4989
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
The ORF is followed by the ADH1 transcription terminator. Please see secondary publication PMID: 22941627 for more information about this plasmid.
growth strain :
Yeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 marker
origin :
37
pi :
alt_names
SUMO
cloning
clone_methodRestriction Enzyme
cloning_site_3SphI
cloning_site_5SacI
promoterCUP1
sequencing_primer_3M13rev
sequencing_primer_5M13fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDR510W
geneSMT3
id852122
genbank_ids
mutation
nameSMT3
shRNA_sequence
size991
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tag7xHis
resistance markers :
3522
tags :
High Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA