This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
YIp204-PADH1-atTIR1-9myc
catalog :
99532
citations: 2
Reference
Gonz xe1 lez L, Kolbin D, Trahan C, Jeronimo C, Robert F, Oeffinger M, et al. Adaptive partitioning of a gene locus to the nuclear envelope in Saccharomyces cerevisiae is driven by polymer-polymer phase separation. Nat Commun. 2023;14:1135 pubmed publisher
John Peter A, Petrungaro C, Peter M, Kornmann B. METALIC reveals interorganelle lipid flux in live cells by enzymatic mass tagging. Nat Cell Biol. 2022;24:996-1004 pubmed publisher
product information
Catalog Number :
99532
Product Name :
YIp204-PADH1-atTIR1-9myc
article :
doi
id28189939
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneYIplac204
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Digest this vector with EcoRV for integration into TRP1. Addgene NGS results found a single nucleotide change compared to NM_116163.4 that results in T591I within the TIR1 translation. The protein is noted by the depositing laboratory to be functional in vivo
growth strain :
S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XmaI
cloning_site_5EcoRI
promoterADH1
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1
geneTIR1
id825473
genbank_ids
mutation
nameTIR1
shRNA_sequence
size2909
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tag9xMyc
plasmid copy :
The TIR1 gene was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
resistance markers :
3522
tags :
High Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA