This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRS303-PADH1-AFB2
catalog :
99530
citations: 1
Reference
Mehta G, Ball D, Eriksson P, Chereji R, Clark D, McNally J, et al. Single-Molecule Analysis Reveals Linked Cycles of RSC Chromatin Remodeling and Ace1p Transcription Factor Binding in Yeast. Mol Cell. 2018;72:875-887.e9 pubmed publisher
product information
Catalog Number :
99530
Product Name :
pRS303-PADH1-AFB2
article :
doi
id28189939
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepRS303
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Digest vector with NheI for integration into HIS3
growth strain :
S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EagI
cloning_site_5EcoRI
promoterADH1
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT3G26810, auxin signaling F-box 2
geneAFB2
id822296
genbank_ids
mutation
nameAFB2
shRNA_sequence
size2804
species
3702
Arabidopsis thaliana
tags
plasmid copy :
The AFB2 gene was a kind gift from Jennifer L. Nemhauser (University of Washington, Seattle, USA).
resistance markers :
3522
tags :
High Copy
terms :
HIS3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA