This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHyg-AID*-6HA
catalog :
99520
citations: 2
Reference
Mattingly M, Seidel C, Muñoz S, Hao Y, Zhang Y, Wen Z, et al. Mediator recruits the cohesin loader Scc2 to RNA Pol II-transcribed genes and promotes sister chromatid cohesion. Curr Biol. 2022;32:2884-2896.e6 pubmed publisher
Morawska M, Ulrich H. An expanded tool kit for the auxin-inducible degron system in budding yeast. Yeast. 2013;30:341-51 pubmed publisher
product information
Catalog Number :
99520
Product Name :
pHyg-AID*-6HA
article :
doi10.1002/yea.2967
id28189938
pubmed_id23836714
bacterial resistance :
Ampicillin
cloning :
backbonepFA6a derivative
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
PCR template for yeast tagging cassette
growth strain :
C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae
origin :
37
pi :
alt_names
AID (Auxin-inducible degron)
AXR3
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3TCGATGAATTCGAGCTCG
sequencing_primer_5CGTACGCTGCAGGTCGAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G04250, AUXIN RESISTANT 3, AtIAA17, F19P19.31, F19P19_31, IAA17, indole-3-acetic acid inducible 17
geneAXR3
id839568
genbank_ids
mutation
nameIAA17(71-116)
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tag6xHA
plasmid copy :
The parent vector encoding AID was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
resistance markers :
3522
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA