This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJC20cdc8.A18T
catalog :
99360
citations: 1
Reference
Johnson C, Brooker H, Gyamfi I, O Brien J, Ashley B, Brazier J, et al. Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on the stability and function of the actin-tropomyosin copolymer. Biochem Biophys Res Commun. 2018;506:339-346 pubmed publisher
product information
Catalog Number :
99360
Product Name :
pJC20cdc8.A18T
article :
doi10.1016/j.bbrc.2017.10.109
id28192205
pubmed_id29080743
bacterial resistance :
Ampicillin
cloning :
backbonepJC20
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5T7 F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSPAC27F1.02c, fus4
genecdc8
id2541940
genbank_ids
mutationAlanine 18 to Threonine
namecdc8
shRNA_sequence
size486
species
4896
Schizosaccharomyces pombe
tags
resistance markers :
1963
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA