STARR-seq luciferase validation vector_ORI_CMV | Addgene 99312 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

STARR-seq luciferase validation vector_ORI_CMV

catalog :

99312

citations: 2

Reference
Præstholm S, Correia C, Goitea V, Siersbæk M, Jørgensen M, Havelund J, et al. Impaired glucocorticoid receptor expression in liver disrupts feeding-induced gene expression, glucose uptake, and glycogen storage. Cell Rep. 2021;37:109938 pubmed publisher
Muerdter F, Boryń Ł, Woodfin A, Neumayr C, Rath M, Zabidi M, et al. Resolving systematic errors in widely used enhancer activity assays in human cells. Nat Methods. 2018;15:141-149 pubmed publisher

product information

Catalog Number :

99312

Product Name :

STARR-seq luciferase validation vector_ORI_CMV

article :

doi	10.1038/nmeth.4534
id	28189890
pubmed_id	29256496

bacterial resistance :

Ampicillin

cloning :

backbone

STARR-seq luciferase validation vector_ORI

backbone_mutation

backbone_origin

Promega

backbone_size

3977

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

STARR-seq Luciferase validation vector

growth notes :

This vector has the CMV enhancer cloned downstream of the reporter gene; The origin of replication (ORI) substitutes the core promoter . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590

growth strain :

Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

CMV enhancer

shRNA_sequence

size

654

species

9606

Homo sapiens