This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCW57.1-DUX4-WT
catalog :
99282
citations: 1
Reference
Jagannathan S, Shadle S, Resnick R, Snider L, Tawil R, van der Maarel S, et al. Model systems of DUX4 expression recapitulate the transcriptional profile of FSHD cells. Hum Mol Genet. 2016;25:4419-4431 pubmed publisher
product information
Catalog Number :
99282
Product Name :
pCW57.1-DUX4-WT
article :
doi10.1093/hmg/ddw271
id28189884
pubmed_id28171552
bacterial resistance :
Ampicillin
cloning :
backbonepCW57.1
backbone_mutation
backbone_origin
backbone_size9354
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)
growth temp :
We used Stbl3 cells (recombination deficient)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NheI
promoterTRE
sequencing_primer_3agggctgccttggaaaag
sequencing_primer_5tcgcctggagaattggc
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesDUX4L
geneDUX4
id100288687
genbank_ids
mutation
nameDUX4
shRNA_sequence
size1275
species
9606
Homo sapiens
tags
resistance markers :
520
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA