pINDUCER22-MKK6E-Flag | Addgene 99259 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pINDUCER22-MKK6E-Flag

catalog :

99259

citations: 1

Reference
Adam A, Lowery A, Martino N, Alsaffar H, Vincent P. Src Family Kinases Modulate the Loss of Endothelial Barrier Function in Response to TNF-α: Crosstalk with p38 Signaling. PLoS ONE. 2016;11:e0161975 pubmed publisher

product information

Catalog Number :

99259

Product Name :

pINDUCER22-MKK6E-Flag

article :

doi	10.1371/journal.pone.0161975
id	23831
pubmed_id	27603666

bacterial resistance :

Ampicillin

cloning :

backbone

pINDUCER/22

backbone_mutation

backbone_origin

Dr. Thomas Westbrook, Baylor College of Medicine

backbone_size

10437

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Contains an IRES-GFP insert

growth strain :

Lentiviral vector encoding a doxycycline-inducible Flag-tagged constitutively active MKK6.

origin :

pi :

alt_names

Dual specificity mitogen-activated protein kinase kinase 6 (active)

MKK6(glu)

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	TRE2
sequencing_primer_3	CCACTTTGTACAAGAAAGCTGGGT
sequencing_primer_5	GTTTGTACAAAAAAGCAGGC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3
gene	MAP2K6
id	5608

genbank_ids

U39656

NM_002758.3

mutation

S207E T211E

name

MKK6E

shRNA_sequence

size

1050

species

9606

Homo sapiens

tags

location	N terminal on insert
tag	Flag

plasmid copy :

MKK6E was from Addgene plasmid #13518. pINDUCER/22 vector was from Dr. Thomas Westbrook, Baylor College of Medicine: Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, et al. The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo. Proceedings of the National Academy of Sciences of the United States of America. 2011;108(9):3665 70. Epub 2011/02/11. pmid:21307310; PubMed Central PMCID: PMC3048138.

resistance markers :

3507

3508

tags :

Unknown