pAD-LyseR (in lacZ deficient strain) | Addgene 99245 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

pAD-LyseR (in lacZ deficient strain)

catalog :

99245

citations: 1

Reference
Didovyk A, Tonooka T, Tsimring L, Hasty J. Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression. ACS Synth Biol. 2017;6:2198-2208 pubmed publisher

product information

Catalog Number :

99245

Product Name :

pAD-LyseR (in lacZ deficient strain)

article :

doi	10.1021/acssynbio.7b00253
id	28191732
pubmed_id	28795570

bacterial resistance :

Ampicillin

cloning :

backbone

pPro24

backbone_mutation

backbone_origin

Jay Keasling lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

pAD-LyseR plasmid in BL21-Gold-dLac (DE3) strain is used for producing autolysate for in vitro transcription-translation reactions without background beta-galactosidase activity.

growth strain :

For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

growth temp :

Use 24% glycerol (w/v) in glycerol stocks to avoid cell lysis upon a freeze-thaw cycle.

inserts :

BL21-Gold-dLac (DE3)

origin :

pi :

alt_names

phage lambda endolysin

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	beta-lactamase promoter
sequencing_primer_3	CCG GGA GCT GCA TGT GTC AGA GG
sequencing_primer_5	CCACATAGCAGAACTTTAAAAGTGCT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	lambdap75
gene	R
id	2703480

genbank_ids

mutation

name

phage lambda gene R

shRNA_sequence

size

474

species

32644

Other

phage lambda