This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAD-LyseR
catalog :
99244
citations: 2
Reference
Niño Sánchez J, Chen L, de Souza J, Mosquera S, Stergiopoulos I. Targeted Delivery of Gene Silencing in Fungi Using Genetically Engineered Bacteria. J Fungi (Basel). 2021;7: pubmed publisher
Didovyk A, Tonooka T, Tsimring L, Hasty J. Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression. ACS Synth Biol. 2017;6:2198-2208 pubmed publisher
product information
Catalog Number :
99244
Product Name :
pAD-LyseR
article :
doi10.1021/acssynbio.7b00253
id28191732
pubmed_id28795570
bacterial resistance :
Ampicillin
cloning :
backbonepPro24
backbone_mutation
backbone_originJay Keasling lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
pAD-LyseR plasmid in BL21-Gold (DE3) strain is used for producing autolysate for in vitro transcription-translation reactions.
growth strain :
For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)
growth temp :
Use 24% glycerol (w/v) in glycerol stocks to avoid cell lysis upon a freeze-thaw cycle.
inserts :
BL21-Gold (DE3)
origin :
37
pi :
alt_names
phage lambda endolysin
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterbeta-lactamase promoter
sequencing_primer_3CCG GGA GCT GCA TGT GTC AGA GG
sequencing_primer_5CCACATAGCAGAACTTTAAAAGTGCT
site_3_destroyed
site_5_destroyed
entrez_gene
aliaseslambdap75
geneR
id2703480
genbank_ids
mutation
namephage lambda gene R
shRNA_sequence
size474
species
32644
Other
phage lambda
tags
resistance markers :
1765
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA