This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCI-Neo-HKIImut
catalog :
99240
citations: 1
| Reference |
|---|
Mathupala S, Heese C, Pedersen P. Glucose catabolism in cancer cells. The type II hexokinase promoter contains functionally active response elements for the tumor suppressor p53. J Biol Chem. 1997;272:22776-80 pubmed
|
product information
Catalog Number :
99240
Product Name :
pCI-Neo-HKIImut
article :
| doi | |
| id | 28189860 |
| pubmed_id | 9278438 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pCI-Neo | |
| backbone_mutation | HKII cDNA (cloned from rat AS-30D hepatoma) inserted in MCS of vector between EcoRI and XbaI sites. A Kozak sequence was for enhanced translation initiation. | |
| backbone_origin | Promega | |
| backbone_size | 5472 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Addgene's QC result found 4 additional mutations (V192A, V378A, M555V, and E699G), that are unexpected; however, the depositor notes that they are away from the catalytic site (and the catalytic site is active).
growth strain :
Type II Hexokinase expressed in AS-30D rat hepatoma cell line
origin :
37
pi :
|
resistance markers :
| 2310 |
tags :
High Copy
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments