This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUB6-CDC34-HA (pRKB261)
catalog :
99145
citations: 1
Reference
Feng Q, Jagannathan S, Bradley R. The RNA Surveillance Factor UPF1 Represses Myogenesis via Its E3 Ubiquitin Ligase Activity. Mol Cell. 2017;67:239-251.e6 pubmed publisher
product information
Catalog Number :
99145
Product Name :
pUB6-CDC34-HA (pRKB261)
article :
doi10.1016/j.molcel.2017.05.034
id28191546
pubmed_id28669802
bacterial resistance :
Ampicillin
cloning :
backbonepUB6/V5-His A
backbone_mutation
backbone_originThermo Fisher Scientific
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
A CDC34 sequence-verified cDNA clone was ordered from Dharmacon (clone ID 4103120). The CDC34 cDNA was cut out with restriction enzymes EcoRI/XhoI (NEB) and cloned into the mammalian expression vector pUB6/V5-His A (Thermo Fisher Scientific) via ligation. A C-terminal HA-tag was introduced via PCR primer overhangs to generate the pUB6-CDC34-HA construct for transfection.
growth strain :
Mammalian expression construct to express HA-tagged CDC34
origin :
37
pi :
alt_names
cell division cycle 34
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoterpTight
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5TCAGTGTTAGACTAGTAAATTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesE2-CDC34, UBC3, UBCH3, UBE2R1
geneCDC34
id997
genbank_ids
NM_004359.1
NP_004350.1
mutation
nameCDC34
shRNA_sequence
size1500
species
9606
Homo sapiens
tags
locationC terminal on insert
tagHA
resistance markers :
3071
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA