This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pADSL-EGFP
catalog :
99109
citations: 1
Reference
An S, Kumar R, Sheets E, Benkovic S. Reversible compartmentalization of de novo purine biosynthetic complexes in living cells. Science. 2008;320:103-6 pubmed publisher
product information
Catalog Number :
99109
Product Name :
pADSL-EGFP
article :
doi10.1126/science.1152241
id28189825
pubmed_id18388293
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutationA206K, L221K, and F223R mutations in EGFP gene to minimize dimerization
backbone_originClontech
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses human ADSL-EGFP fusion in mammalian cells
origin :
37
pi :
alt_names
ASL
adenylosuccinate lyase
cloning
clone_methodRestriction Enzyme
cloning_site_3XmaI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3
sequencing_primer_5CGCAAATGGGCGGTAGGCGTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAMPS, ASASE, ASL
geneADSL
id158
genbank_ids
NM_000026
mutation
nameADSL
shRNA_sequence
size1452
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP (enhanced green fluorescent protein)
resistance markers :
3618
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA