This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCEP4-FLAG-CXCR4-VN
catalog :
98964
citations: 3
Reference
Gill K, Mehta K, Heredia J, Krishnamurthy V, Zhang K, Procko E. Multiple mechanisms of self-association of chemokine receptors CXCR4 and CCR5 demonstrated by deep mutagenesis. J Biol Chem. 2023;299:105229 pubmed publisher
Gill K, Mehta K, Heredia J, Krishnamurthy V, Zhang K, Procko E. Multiple mechanisms of self-association of chemokine receptors CXCR4 and CCR5 demonstrated by deep mutagenesis. bioRxiv. 2023;: pubmed publisher
Heredia J, Park J, Brubaker R, Szymanski S, Gill K, Procko E. Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning. J Immunol. 2018;200:3825-3839 pubmed publisher
product information
Catalog Number :
98964
Product Name :
pCEP4-FLAG-CXCR4-VN
article :
doi10.4049/jimmunol.1800343
id28189812
pubmed_id29678950
bacterial resistance :
Ampicillin
cloning :
backbonepCEP4
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
FLAG-tagged human CXCR4 fused to N-terminus of split Venus
origin :
37
pi :
alt_names
CD184
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NheI
promoterCMV
sequencing_primer_3EBV_rev
sequencing_primer_5CMV_fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1
geneCXCR4
id7852
genbank_ids
NM_003467
mutationFull-length, wildtype
nameCXCR4
shRNA_sequence
size1611
species
9606
Homo sapiens
tags
locationN terminal on insert
tagSignal/leader sequence from Influenza A virus HA protein
locationN terminal on insert
tagFLAG (dykdddd) epitope tag
locationC terminal on insert
tagVN: Venus (I152L) N-terminal residues 1-154
resistance markers :
3492
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA