gRNA-CXCR4(90) | Addgene 98962 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

gRNA-CXCR4(90)

catalog :

98962

citations: 1

Reference
Heredia J, Park J, Brubaker R, Szymanski S, Gill K, Procko E. Mapping Interaction Sites on Human Chemokine Receptors by Deep Mutational Scanning. J Immunol. 2018;200:3825-3839 pubmed publisher

product information

Catalog Number :

98962

Product Name :

gRNA-CXCR4(90)

article :

doi	10.4049/jimmunol.1800343
id	28189812
pubmed_id	29678950

bacterial resistance :

Kanamycin

cloning :

backbone

pCR-Blunt II-TOPO

backbone_mutation

Insert was cloned into the gRNA_Cloning Vector (Addgene Plasmid #41824) from the George Church laboratory.

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

growth strain :

Mammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2

origin :

pi :

alt_names

CD184

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	U6
sequencing_primer_3
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS, WHIMS1
gene	CXCR4
id	7852

genbank_ids

NM_003467

mutation

Encodes a gRNA for CRISPR-mediated targeting of CXCR4.

name

CXCR4

shRNA_sequence

size

species

9606

Homo sapiens