pRL_GI_PCB28 | Addgene 98745 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRL_GI_PCB28

catalog :

98745

citations: 1

Reference
Hochrein L, Machens F, Messerschmidt K, Mueller Roeber B. PhiReX: a programmable and red light-regulated protein expression switch for yeast. Nucleic Acids Res. 2017;45:9193-9205 pubmed publisher

product information

Catalog Number :

98745

Product Name :

pRL_GI_PCB28

article :

doi	10.1093/nar/gkx610
id	28189770
pubmed_id	28911120

bacterial resistance :

Kanamycin

cloning :

backbone

pL0C_4-R

backbone_mutation

Insertion of a TRP marker, Insertion of a genome integration cassette for the his1- 200 locus

backbone_origin

backbone_size

5864

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

Synthetic Biology

growth strain :

For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1- 200 locus. Encodes constitutively expressed HY1 and PcyA.

origin :

pi :

alt_names

HY1

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	PGK1
sequencing_primer_3	GTTTAGTAGAACCTCGTG
sequencing_primer_5	CGTAAAGGATGGGGAAAGAGA
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT2G26670, ARABIDOPSIS THALIANA HEME OXYGENASE 1, ATHO1, F18A8.4, F18A8_4, GENOMES UNCOUPLED 2, GUN2, HEME OXYGENASE, HEME OXYGENASE 1, HEME OXYGENASE 6, HO1, HY1, HY6, PLASTID HEME OXYGENASE, REVERSAL OF THE DET PHENOTYPE 4
gene	TED4
id	817208

genbank_ids

AB021858

mutation

name

heme oxygenase 1

shRNA_sequence

size

849

species

3702

Arabidopsis thaliana