This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MBP-FUS_FL_WT
catalog :
98651
citations: 17
Reference
Hernández Candia C, Brady B, Harrison E, Tucker C. A platform to induce and mature biomolecular condensates using chemicals and light. Nat Chem Biol. 2024;: pubmed publisher
Guo L, Mann J, Mauna J, Copley K, Wang H, Rubien J, et al. Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. bioRxiv. 2023;: pubmed publisher
F xe9 lix S, Laurents D, Oroz J, Cabrita E. Fused in sarcoma undergoes cold denaturation: Implications for phase separation. Protein Sci. 2023;32:e4521 pubmed publisher
Kamal M, Tokmakjian L, Knox J, Mastrangelo P, Ji J, Cai H, et al. A spatiotemporal reconstruction of the C. elegans pharyngeal cuticle reveals a structure rich in phase-separating proteins. elife. 2022;11: pubmed publisher
Kamagata K, Ariefai M, Takahashi H, Hando A, Subekti D, Ikeda K, et al. Rational peptide design for regulating liquid-liquid phase separation on the basis of residue-residue contact energy. Sci Rep. 2022;12:13718 pubmed publisher
Murthy A, Tang W, Jovic N, Janke A, Seo D, Perdikari T, et al. Molecular interactions contributing to FUS SYGQ LC-RGG phase separation and co-partitioning with RNA polymerase II heptads. Nat Struct Mol Biol. 2021;28:923-935 pubmed publisher
Owen I, Yee D, Wyne H, Perdikari T, Johnson V, Smyth J, et al. The oncogenic transcription factor FUS-CHOP can undergo nuclear liquid-liquid phase separation. J Cell Sci. 2021;134: pubmed publisher
Birsa N, Ule A, Garone M, Tsang B, Mattedi F, Chong P, et al. FUS-ALS mutants alter FMRP phase separation equilibrium and impair protein translation. Sci Adv. 2021;7: pubmed publisher
Lin Y, Kumar M, Ramesh N, Anderson E, Nguyen A, Kim B, et al. Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway. Nat Neurosci. 2021;24:1077-1088 pubmed publisher
Kamagata K, Chiba R, Kawahata I, Iwaki N, Kanbayashi S, Maeda K, et al. Characterization of design grammar of peptides for regulating liquid droplets and aggregates of FUS. Sci Rep. 2021;11:6643 pubmed publisher
Hernández Candia C, Pearce S, Tucker C. A modular tool to query and inducibly disrupt biomolecular condensates. Nat Commun. 2021;12:1809 pubmed publisher
Freibaum B, Messing J, Yang P, Kim H, Taylor J. High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate. J Cell Biol. 2021;220: pubmed publisher
Perdikari T, Murthy A, Ryan V, Watters S, Naik M, Fawzi N. SARS-CoV-2 nucleocapsid protein phase-separates with RNA and with human hnRNPs. EMBO J. 2020;:e106478 pubmed publisher
Babinchak W, Dumm B, Venus S, Boyko S, Putnam A, Jankowsky E, et al. Small molecules as potent biphasic modulators of protein liquid-liquid phase separation. Nat Commun. 2020;11:5574 pubmed publisher
Owen I, Rhoads S, Yee D, Wyne H, Gery K, Hannula I, et al. The prion-like domain of Fused in Sarcoma is phosphorylated by multiple kinases affecting liquid- and solid-phase transitions. Mol Biol Cell. 2020;31:2522-2536 pubmed publisher
Perdikari T, Murthy A, Ryan V, Watters S, Naik M, Fawzi N. SARS-CoV-2 nucleocapsid protein undergoes liquid-liquid phase separation stimulated by RNA and partitions into phases of human ribonucleoproteins. bioRxiv. 2020;: pubmed publisher
Burke K, Janke A, Rhine C, Fawzi N. Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II. Mol Cell. 2015;60:231-41 pubmed publisher
product information
Catalog Number :
98651
Product Name :
MBP-FUS_FL_WT
article :
doi10.1016/j.molcel.2015.09.006
id28189756
pubmed_id26455390
bacterial resistance :
Kanamycin
cloning :
backbonepTHMT
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses MBP-tagged full length FUS
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS
geneFUS
id2521
genbank_ids
mutation
nameFUS
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6xHis-MBP
resistance markers :
3468
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA