This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
BRAF_pLX307
catalog :
98322
citations: 2
Reference
Skalamera D, Stevenson A, Ehmann A, Ainger S, Lanagan C, Sturm R, et al. Melanoma mutations modify melanocyte dynamics in co-culture with keratinocytes or fibroblasts. J Cell Sci. 2019;132: pubmed publisher
Rosenbluh J, Mercer J, Shrestha Y, Oliver R, Tamayo P, Doench J, et al. Genetic and Proteomic Interrogation of Lower Confidence Candidate Genes Reveals Signaling Networks in β-Catenin-Active Cancers. Cell Syst. 2016;3:302-316.e4 pubmed publisher
product information
Catalog Number :
98322
Product Name :
BRAF_pLX307
article :
doi10.1016/j.cels.2016.09.001
id24025
pubmed_id27684187
bacterial resistance :
Ampicillin
cloning :
backbonepLX307
backbone_mutation
backbone_originDavid Root (Addgene plasmid # 41392)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Lentiviral expression of BRAF
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterE1Fa
sequencing_primer_3GTGGATACGCTGCTTTAATGCCTT
sequencing_primer_5TTCTTCCATTTCAGGTGTCGTGAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
geneBRAF
id673
genbank_ids
mutation
nameBRAF
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagV5
resistance markers :
184
3458
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA