pmEos2-Linker_PCNA-MutC | Addgene 98272 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pmEos2-Linker_PCNA-MutC

catalog :

98272

citations: 1

Reference
Zessin P, Sporbert A, Heilemann M. PCNA appears in two populations of slow and fast diffusion with a constant ratio throughout S-phase in replicating mammalian cells. Sci Rep. 2016;6:18779 pubmed publisher

product information

Catalog Number :

98272

Product Name :

pmEos2-Linker_PCNA-MutC

article :

doi	10.1038/srep18779
id	28189715
pubmed_id	26758689

bacterial resistance :

Kanamycin

cloning :

backbone

backbone_mutation

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Plasmid encodes a second insert: mEos2 (from Lobophyllia hemprichii). A glycine-rich linker sequence (GEGQGQGQGPGRGYAYRS), which was reported to be necessary for cell line generation, was inserted as a spacer between mEos2 and PCNA.

growth strain :

for low level expression of mEos2-PCNA in mammalian cells

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ATLD2
gene	PCNA
id	5111

genbank_ids

mutation

Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (Original: GATGCTGTTGTAATTTCCTGT)

name

PCNA

shRNA_sequence

size

782

species

9606

Homo sapiens