This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBa-LSS-GFP-LDLR wt
catalog :
98184
citations: 4
Reference
Chen Y, Wu X, Zhang J, Pan G, Wang X, Guo X, et al. Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. EMBO Rep. 2022;:e53373 pubmed publisher
Ho W, Chang J, Lim K, Cazenave Gassiot A, Nguyen A, Foo J, et al. TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination. J Cell Biol. 2021;220: pubmed publisher
Oleaga C, Hay J, Gurcan E, David L, Mueller P, Tavori H, et al. Insights into the kinetics and dynamics of the furin-cleaved form of PCSK9. J Lipid Res. 2021;62:100003 pubmed publisher
Jenkins B, Decker H, Bentley M, Luisi J, Banker G. A novel split kinesin assay identifies motor proteins that interact with distinct vesicle populations. J Cell Biol. 2012;198:749-61 pubmed publisher
product information
Catalog Number :
98184
Product Name :
pBa-LSS-GFP-LDLR wt
article :
doi10.1083/jcb.201205070
id28189708
pubmed_id22908316
bacterial resistance :
Ampicillin
cloning :
backbonepBa-eGFP
backbone_mutation
backbone_origin
backbone_size6755
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.
origin :
37
pi :
alt_names
human low density lipoprotein receptor
cloning
clone_methodRestriction Enzyme
cloning_site_3RsrII
cloning_site_5AvrII-vector, NheI-insert
promoterChicken Beta Actin
sequencing_primer_3GATGAGTTTGGACAAACCAC
sequencing_primer_5TTCGGCTTCTGGCGTGTGAC
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesFH, FHC, FHCL1, LDLCQ2
geneLDLR
id3949
genbank_ids
NM_000527
mutationnone
nameLDLR
shRNA_sequence
size3326
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
locationN terminal on insert
tagLDLR signal sequence, N-term of GFP so GFP remains on mature protein when ss is cleaved
resistance markers :
1566
3547
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA