This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-PTEN-3'UTR
catalog :
97204
citations: 1
Reference
Kerwin J, Khan I, Iorns E, Tsui R, Denis A, Perfito N, et al. Replication Study: A coding-independent function of gene and pseudogene mRNAs regulates tumour biology. elife. 2020;9: pubmed publisher
product information
Catalog Number :
97204
Product Name :
pCMV-PTEN-3'UTR
article :
doi10.7554/eLife.51019
id28189650
pubmed_id32314732
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-MCS
backbone_mutation
backbone_originAgilent
backbone_size4500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
pCMV-PTEN-3'UTR was created by sub-cloning partial fragments of PTEN 3'UTR from HeLa gDNA using Gibson Assembly. The cloned insert was confirmed by DNA sequencing.
growth strain :
Expresses PTEN 3'UTR in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3hGH polyA Reverse, TAGAAGGACACCTAGTCAGA
sequencing_primer_5CMV Forward or B-globin Forward, ATTCTGAGTCCAAGCTAGGC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1
genePTEN
id5728
genbank_ids
U93051
mutation
namePTEN 3' UTR
shRNA_sequence
size3393
species
9606
Homo sapiens
tags
location
tagnone
resistance markers :
3441
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA