This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
[UBC][firefly luciferase][target cloning][renilla luciferase]
catalog :
97190
citations: 1
Reference
Adamala K, Martin Alarcon D, Boyden E. Programmable RNA-binding protein composed of repeats of a single modular unit. Proc Natl Acad Sci U S A. 2016;113:E2579-88 pubmed publisher
product information
Catalog Number :
97190
Product Name :
[UBC][firefly luciferase][target cloning][renilla luciferase]
article :
doi10.1073/pnas.1519368113
id18525
pubmed_id27118836
bacterial resistance :
Ampicillin
cloning :
backbonepCI
backbone_mutationmutated to remove BsaI, original promoter replaced with UBC promoter
backbone_originPromega
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Synthetic Biology
growth notes :
Target cloning is a HindIII-AscI site for inserting recognition sequences for Pum mediated translation activation of the downstream gene. This plasmid corresponds to that shown in Figure 6A.
growth strain :
Reporter for translation activation via Pum.
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterUBC (ubiquitin C)
sequencing_primer_3GTATCTTATCATGTCTGCTCGAAG
sequencing_primer_5TAATACGACTCACTATAGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameFirefly Luciferase
shRNA_sequence
size1686
species
32644
Other
firefly
tags
location
tagnone
alt_names
cloning
entrez_gene
genbank_ids
mutation
nameRenilla Luciferase
shRNA_sequence
size936
species
32644
Other
sea pansy
tags
resistance markers :
50
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA