This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
[UBC][EIF4e][GG cloning]
catalog :
97189
citations: 1
Reference
Adamala K, Martin Alarcon D, Boyden E. Programmable RNA-binding protein composed of repeats of a single modular unit. Proc Natl Acad Sci U S A. 2016;113:E2579-88 pubmed publisher
product information
Catalog Number :
97189
Product Name :
[UBC][EIF4e][GG cloning]
article :
doi10.1073/pnas.1519368113
id18525
pubmed_id27118836
bacterial resistance :
Ampicillin
cloning :
backbonepCI
backbone_mutationmutated to remove BsaI, original promoter replaced with UBC promoter
backbone_originPromega
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Synthetic Biology
growth notes :
Experiments using this plasmid are shown in Figure 6 of our paper. [GG] is a Golden Gate cloning site, a BsaI enzyme restriction site with sticky ends CGAG___ACGC, as described in cloning scheme in Figure S1.
growth strain :
EIF4e plasmid with Pum cloning site
origin :
37
pi :
alt_names
AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterUBC (ubiquitin C)
sequencing_primer_3GTATCTTATCATGTCTGCTCGAAG
sequencing_primer_5TAATACGACTCACTATAGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E
geneEIF4E
id1977
genbank_ids
NM_001968
mutation
nameEIF4E variant 1
shRNA_sequence
size651
species
9606
Homo sapiens
tags
locationC terminal on insert
tagempty Pum cloning site
resistance markers :
50
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA