This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBS-AMVpl- WT-nAchR
catalog :
97187
citations: 1
Reference
Labarca C, Nowak M, Zhang H, Tang L, Deshpande P, Lester H. Channel gating governed symmetrically by conserved leucine residues in the M2 domain of nicotinic receptors. Nature. 1995;376:514-6 pubmed
product information
Catalog Number :
97187
Product Name :
pBS-AMVpl- WT-nAchR
article :
doi10.1038/376514a0
id2481
pubmed_id7637783
bacterial resistance :
Ampicillin
cloning :
backbonepBluescript KS+
backbone_mutationNA
backbone_originStratagene
backbone_size2964
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co
origin :
37
pi :
alt_names
WTnAchR
cloning
clone_methodRestriction Enzyme
cloning_site_3Xba1
cloning_site_5Bcl1
promoterT7
sequencing_primer_3pBSKSreverse(+) primer
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAchr-3, Acrg
geneChrng
id11449
genbank_ids
P04760.1
mutationWT
nameGamma nicotinic acetylcholine receptor
shRNA_sequence
size1560
species
10090
Mus musculus
tags
plasmid copy :
Lester Lab
resistance markers :
288
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA