pYFP-NEAT1_IS_RT | Addgene 97091 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pYFP-NEAT1_IS_RT

catalog :

97091

citations: 1

Reference
Li R, Harvey A, Hodgetts S, Fox A. Functional dissection of NEAT1 using genome editing reveals substantial localization of the NEAT1_1 isoform outside paraspeckles. RNA. 2017;23:872-881 pubmed publisher

product information

Catalog Number :

97091

Product Name :

pYFP-NEAT1_IS_RT

article :

doi	10.1261/rna.059477.116
id	25680
pubmed_id	28325845

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

backbone_origin

Clontech

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

CRISPR

growth notes :

The entire repair template is synthesized and then cloned into the MluI site. Original EGFP was mutated to EYFP using mutation: L65F, T66G, V69L, S73A, T204Y. Also see original publication for mutations on the repair template.

growth strain :

Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Unknown
cloning_site_5	Unknown
promoter	CMV
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	LINC00084, NCRNA00084, TP53LC15, TncRNA, VINC
gene	NEAT1
id	283131

genbank_ids

mutation

name

NEAT1_IS_RT

shRNA_sequence

size

species

9606

Homo sapiens