pMDC32-FIT2-FLL[157-159]AAA | Addgene 96991 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMDC32-FIT2-FLL[157-159]AAA

catalog :

96991

citations: 1

Reference
Cai Y, McClinchie E, Price A, Nguyen T, Gidda S, Watt S, et al. Mouse fat storage-inducing transmembrane protein 2 (FIT2) promotes lipid droplet accumulation in plants. Plant Biotechnol J. 2017;15:824-836 pubmed publisher

product information

Catalog Number :

96991

Product Name :

pMDC32-FIT2-FLL[157-159]AAA

article :

doi	10.1111/pbi.12678
id	28189627
pubmed_id	27987528

bacterial resistance :

Kanamycin

cloning :

backbone

pMDC32

backbone_mutation

backbone_origin

backbone_size

11752

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Plant Expression

growth notes :

The sequences and maps of pMDC vectors can be obtained from the following website. http://www.botinst.uzh.ch/en/research/development/grossnik/vectors/MarkdGatewayVectors.html Please note that mutations E2D and I130V in FIT2 were found during Addgene's quality control process. The depositor noted that these mutations do NOT affect plasmid function.

growth strain :

Express mouse FIT2 gene in plants.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	PacI
cloning_site_5	AscI
promoter	35S
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	D930001I22Rik, Fit2
gene	Fitm2
id	228859

genbank_ids

mutation

Amino acid residues 157-159 were mutated (FLL[157-159]AAA). Mutations E2D and I130V also present (see depositor comments below)

name

MmFIT2

shRNA_sequence

size

789

species

10090

Mus musculus