This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMGF130
catalog :
96971
citations: 1
Reference
Gu M, LaJoie D, Chen O, von Appen A, Ladinsky M, Redd M, et al. LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells. Proc Natl Acad Sci U S A. 2017;114:E2166-E2175 pubmed publisher
product information
Catalog Number :
96971
Product Name :
pMGF130
article :
doi10.1073/pnas.1613916114
id28189625
pubmed_id28242692
bacterial resistance :
Ampicillin
cloning :
backbonepGEM GST vector
backbone_mutation
backbone_origin
backbone_size4800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
S. pombe lem2 knockout cassette with hygro resistance gene
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterN/A
sequencing_primer_3ATCCATAGTGTCGAACCCTTC
sequencing_primer_5CTCGACATCGCTGGAAGCAAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSPAC18G6.10, heh1
genelem2
id2542544
genbank_ids
mutationwhole ORF deletion and replaced with hygro resistance gene
namelem2
shRNA_sequence
size2700
species
4896
Schizosaccharomyces pombe
tags
resistance markers :
3500
2886
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA