This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
WT1 KI gRNA2
catalog :
92313
citations: 1
Reference
Bao X, Lian X, Hacker T, Schmuck E, Qian T, Bhute V, et al. Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions. Nat Biomed Eng. 2016;1: pubmed publisher
product information
Catalog Number :
92313
Product Name :
WT1 KI gRNA2
article :
doi10.1038/s41551-016-0003
id25668
pubmed_id28462012
bacterial resistance :
Ampicillin
cloning :
backboneCRISPR-eGFP
backbone_mutationgRNA1 targeting WT1
backbone_origin
backbone_size9289
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
CRISPR-GFP-gRNA for cutting WT1
origin :
37
pi :
alt_names
AWT1
cloning
clone_methodRestriction Enzyme
cloning_site_3BBSI
cloning_site_5BBSI
promoterU6
sequencing_primer_3CGGGTACCTCTAGAGCCATTT
sequencing_primer_5tttcttgggtagtttgcagtttt
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesAWT1, GUD, NPHS4, WAGR, WIT-2, WT-1, WT33
geneWT1
id7490
genbank_ids
7490
mutation
nameWT1
shRNA_sequenceGGACACTGAACGGTCCCCGA (gRNA shown is without PAM sequence)
size
species
9606
Homo sapiens
tags
location
tagCRISPR GFP
resistance markers :
793
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA