This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
VE-cad-eGFP Donor plasmid
catalog :
92309
citations: 1
Reference
Chang Y, Syahirah R, Oprescu S, Wang X, Jung J, Cooper S, et al. Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells. Biomaterials. 2022;285:121569 pubmed publisher
product information
Catalog Number :
92309
Product Name :
VE-cad-eGFP Donor plasmid
article :
doi10.1002/btm2.10062
id25667
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backboneOct4-2A-eGFP backbone
backbone_mutationLeft and right arms of Oct4 were replaced with VEcad arms
backbone_origin
backbone_size8563
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
This plasmid functions as described in the related publication, but the 5' homology arm is 528bp instead of ~2kbp.
growth strain :
Knock eGFP into human VE-cad locus
origin :
37
pi :
alt_names
CDH5
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI, FseI
cloning_site_5SbfI, AscI
promoter
sequencing_primer_3M13 FWD
sequencing_primer_5M13 RVS
site_3_destroyed
site_5_destroyed
entrez_gene
aliases7B4, CD144
geneCDH5
id1003
genbank_ids
1003
mutationabout 30 a.a. truncated at end of protein
nameVEcad Homologous Recombination Arms
shRNA_sequence
size2000
species
9606
Homo sapiens
tags
resistance markers :
793
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA