This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMCh-646
catalog :
92289
citations: 1
Reference
Chekulaeva M, Mathys H, Zipprich J, Attig J, Colic M, Parker R, et al. miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Nat Struct Mol Biol. 2011;18:1218-26 pubmed publisher
product information
Catalog Number :
92289
Product Name :
pMCh-646
article :
doi10.1038/nsmb.2166
id25662
pubmed_id21984184
bacterial resistance :
Ampicillin
cloning :
backbonepAC5.1-NHA
backbone_mutation
backbone_origin
backbone_size5455
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells
origin :
37
pi :
alt_names
Gawky
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5SbfI
promoterAc5
sequencing_primer_3CCT TAG AAG GCA CAG TCG AGG
sequencing_primer_5ACA CAA AGC CGC TCC ATC AG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG31992, CG11484, CG31992, CG9905, Dmel CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182
genegw
id43808
genbank_ids
NM_166783.2
mutationdGW182 CED: aa 940-1215 with the following point mutations: W942A; W1024A; W1037A; W1051A; W1092A; W1107A; W1114A
namedGW182 CED 7W7A
shRNA_sequence
size840
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagNHA
resistance markers :
3014
3598
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA