This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMCh-695
catalog :
92263
citations: 1
Reference
Chekulaeva M, Mathys H, Zipprich J, Attig J, Colic M, Parker R, et al. miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Nat Struct Mol Biol. 2011;18:1218-26 pubmed publisher
product information
Catalog Number :
92263
Product Name :
pMCh-695
article :
doi10.1038/nsmb.2166
id25662
pubmed_id21984184
bacterial resistance :
Ampicillin
cloning :
backbonepAC5.1-NHA
backbone_mutation
backbone_origin
backbone_size5445
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
Note: Addgene's quality control sequencing finds two additional amino acid residue substitutions (V704A and N799S). The effects of these additional residue changes on protein function are not known.
growth strain :
Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells
origin :
37
pi :
alt_names
Gawky
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5SbfI
promoterAc5
sequencing_primer_3CCT TAG AAG GCA CAG TCG AGG
sequencing_primer_5ACA CAA AGC CGC TCC ATC AG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG31992, CG11484, CG31992, CG9905, Dmel CG31992, GQ182, GW, GW182, Gw, Gw182, anon-EST:Posey205, dGW182, gw182
genegw
id43808
genbank_ids
NM_166783.2
mutationThe following point mutations were introduced: EF960AA; WK967AA; T982A.
namedGW182 PAM2 mut
shRNA_sequence
size4164
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagNHA
resistance markers :
3014
3598
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA