This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMCh-633
catalog :
92253
citations: 1
Reference
Chekulaeva M, Mathys H, Zipprich J, Attig J, Colic M, Parker R, et al. miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs. Nat Struct Mol Biol. 2011;18:1218-26 pubmed publisher
product information
Catalog Number :
92253
Product Name :
pMCh-633
article :
doi10.1038/nsmb.2166
id25662
pubmed_id21984184
bacterial resistance :
Ampicillin
cloning :
backbonepEBG
backbone_mutation
backbone_originAddgene 22227
backbone_size5996
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterEF1A
sequencing_primer_3ACAGGGATTTCTTGTCTCCCAC
sequencing_primer_5atagcatggcctttgcaggg
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneTNRC6C
id57690
genbank_ids
NM_001142640.1
mutationC-terminal effector domain (CED): aa 1369-1690 with the following point mutations: W1445A; W1487A;W1494A; W1504A; W1605A; W1648A; W1659A
nameTNRC6C CED
shRNA_sequence
size969
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
resistance markers :
3014
3598
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA