This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pJOP-HTT-HR65Q
catalog :
92249
citations: 1
Reference
Ooi J, Langley S, Xu X, Utami K, Sim B, Huang Y, et al. Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects. Cell Rep. 2019;26:2494-2508.e7 pubmed publisher
product information
Catalog Number :
92249
Product Name :
pJOP-HTT-HR65Q
article :
doi10.1016/j.celrep.2019.02.008
id25113
pubmed_id30811996
bacterial resistance :
Ampicillin
cloning :
backbonePBHR100A-1 (SBI)
backbone_mutation
backbone_origin
backbone_size7925
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
PrecisionX HR targeting vector
growth strain :
HTT donor DNA with 65Q for homologous recombination-mediated gene modification
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BspQI (destroyed)/SpeI
cloning_site_5NheI/NotI
promoter
sequencing_primer_3TCATTTTGACTCACGCGGTC (-196)
sequencing_primer_5GTTTCGCCACCTCTGACTTG (-588)
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesHD, IT15, LOMARS
geneHTT
id3064
genbank_ids
mutationBarcode of wobble bases to prevent TALEN cleaving donor DNA: CAA CTG CCC CAA CCG CC
nameHTT donor DNA
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
3270
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA