This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
BtuF
catalog :
92209
citations: 1
Reference
Borths E, Locher K, Lee A, Rees D. The structure of Escherichia coli BtuF and binding to its cognate ATP binding cassette transporter. Proc Natl Acad Sci U S A. 2002;99:16642-7 pubmed
product information
Catalog Number :
92209
Product Name :
BtuF
article :
doi10.1073/pnas.262659699
id25653
pubmed_id12475936
bacterial resistance :
Ampicillin
cloning :
backbonepET22b(+)
backbone_mutation
backbone_originNovagen
backbone_size5406
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter
origin :
37
pi :
alt_names
Vitamin B12 Binding Protein
vitamin B12 ABC transporter periplasmic binding protein
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7 Promoter
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5taatacgactcactataggg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb0158, ECK0157, JW0154, yadT
genebtuF
id947574
genbank_ids
947574
mutationBtuF signal sequence removed and uses the PelB signal sequence
nameBtuF
shRNA_sequence
size774
species
tags
locationC terminal on insert
tag6x His Tag
locationN terminal on insert
tagPelB Signal Sequence
resistance markers :
2861
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA