This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
BtuCD
catalog :
92208
citations: 1
Reference
Locher K, Lee A, Rees D. The E. coli BtuCD structure: a framework for ABC transporter architecture and mechanism. Science. 2002;296:1091-8 pubmed
product information
Catalog Number :
92208
Product Name :
BtuCD
article :
doi10.1126/science.1071142
id25652
pubmed_id12004122
bacterial resistance :
Ampicillin
cloning :
backbonepET21b+
backbone_mutationAdded entire expression section of pET19b with BtuC gene into original pET21b+. There are 2 expression regions for BtuC and BtuD each with their own T7 Promoter and Terminator
backbone_originNovagen
backbone_size5221
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expression of BtuCD in E. coli
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterT7 Promoter
sequencing_primer_3tactggatgatgggcggtttt AND ctaACGTCCTGCTTTTAACAATAACCAG
sequencing_primer_5gccgttgagcaccgccgccgcaaggaatgg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb1711, ECK1709, JW1701
genebtuC
id945877
genbank_ids
mutation
namebtuC
shRNA_sequence
size978
species
tags
locationN terminal on insert
tag10x Histidine Tag
locationN terminal on insert
tagEnterokinase Cleavage Site
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7 Promoter
sequencing_primer_3CCGCGCTTAATGCGCCGCTACAGGGCGCGT
sequencing_primer_5CTGCCGCAGAGCTGCCTATTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb1709, ECK1707, JW1699
genebtuD
id945751
genbank_ids
mutation
namebtuD
shRNA_sequence
size747
species
tags
resistance markers :
2861
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA