This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting
catalog :
92139
citations: 1
Reference
Nora E, Goloborodko A, Valton A, Gibcus J, Uebersohn A, Abdennur N, et al. Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Cell. 2017;169:930-944.e22 pubmed publisher
product information
Catalog Number :
92139
Product Name :
pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting
article :
doi10.1016/j.cell.2017.05.004
id28189647
pubmed_id28525758
bacterial resistance :
Ampicillin
cloning :
backbonepEN395 (Addgene #92141)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Mouse Targeting
growth notes :
use with pX330-EN1201 (Cas9 + sgRNA against mouse TIGRE acceptor locus)
growth strain :
Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesGem, MGORS6
geneGMNN
id51053
aliasesDUP, RIS2
geneCDT1
id81620
genbank_ids
mutation
namechromatin licensing and DNA replication factor 1
shRNA_sequence
size
species
9606
Homo sapiens
tags
plasmid copy :
hGeminin and mCherry-Cdt1 were PCR amplified from pRetroX-S2G2M and pRetroX-G1-Red (Clonetech)
resistance markers :
740
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA