U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1 | Addgene 92120 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

catalog :

92120

citations: 3

Reference
Zhang B, Ren Z, Zheng H, Lin M, Chen G, Luo O, et al. CRISPR activation screening in a mouse model for drivers of hepatocellular carcinoma growth and metastasis. iScience. 2023;26:106099 pubmed publisher
Suzuki H, Shibagaki Y, Hattori S, Matsuoka M. C9-ALS/FTD-linked proline-arginine dipeptide repeat protein associates with paraspeckle components and increases paraspeckle formation. Cell Death Dis. 2019;10:746 pubmed publisher
Kulcsár P, Tálas A, Huszár K, Ligeti Z, Tóth E, Weinhardt N, et al. Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Genome Biol. 2017;18:190 pubmed publisher

product information

Catalog Number :

92120

Product Name :

U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

article :

doi	10.1186/s13059-017-1318-8 [pii]
id	28192054
pubmed_id	28985763

bacterial resistance :

Ampicillin

cloning :

backbone

pX330-like

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

growth notes :

U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1 is an altered version of the #61424 and #61423 plasmids from the Feng Zhang lab. This plasmid makes it easier to transiently express the MS2 containing sgRNA and the MS2-P65-HSF1 activator helper complex in cell lines. For detailed information and plasmid usage, please see the associated publication.

growth strain :

Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	EF1a
sequencing_primer_3
sequencing_primer_5	CCTCTGACTTGAGCGTCG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HSTF1
gene	HSF1
id	3297

genbank_ids

mutation

name

MS2-P65-HSF1

shRNA_sequence

size

1428

species

9606

Homo sapiens

100

Synthetic