This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
lentiMPH v2 (Neo)
catalog :
92065
citations: 1
Reference
Barger C, Chee L, Albahrani M, Munoz Trujillo C, Boghean L, Branick C, et al. Co-regulation and function of FOXM1/RHNO1 bidirectional genes in cancer. elife. 2021;10: pubmed publisher
product information
Catalog Number :
92065
Product Name :
lentiMPH v2 (Neo)
article :
doi10.7554/eLife.55070
id28216745
pubmed_id33890574
bacterial resistance :
Ampicillin
cloning :
backbonelentiMPH v2
backbone_mutationRemoved T2A-Hygro by digestion with Bsp1407I and EcoRI. Added T2A-Neo by PCR amplification and ligation into these restriction sites.
backbone_originFeng Zhang lab
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BsiWI
promoterEF1a
sequencing_primer_3
sequencing_primer_5cacatagcgtaaaaggagcaacatag
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHSTF1
geneHSF1
id3297
genbank_ids
mutationN55K in MS2
nameMS2-P65-HSF1_2A_Neo
shRNA_sequence
size2526
species
9606
Homo sapiens
100
Synthetic
tags
resistance markers :
2517
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA