This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFREE
catalog :
92050
citations: 3
Reference
Yan F, Wang J, Zhang S, Lu Z, Li S, Ji Z, et al. CRISPR/FnCas12a-mediated efficient multiplex and iterative genome editing in bacterial plant pathogens without donor DNA templates. PLoS Pathog. 2023;19:e1010961 pubmed publisher
Clifton B, Fariz M, Uechi G, Laurino P. Evolutionary repair reveals an unexpected role of the tRNA modification m1G37 in aminoacylation. Nucleic Acids Res. 2021;49:12467-12485 pubmed publisher
Lauritsen I, Porse A, Sommer M, Nørholm M. A versatile one-step CRISPR-Cas9 based approach to plasmid-curing. Microb Cell Fact. 2017;16:135 pubmed publisher
product information
Catalog Number :
92050
Product Name :
pFREE
article :
doi10.1186/s12934-017-0748-z [pii]
id28191678
pubmed_id28764701
bacterial resistance :
Kanamycin
cloning :
backbonepMAZ-SK
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
CRISPR
growth strain :
Allows inducible expression of gRNAs and Cas9 for plasmid curing.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterptet
sequencing_primer_3TACCGGTTTATTGACTACC
sequencing_primer_5AAAGTCTGGTTATAACC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCas9
shRNA_sequence
size
species
tags
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterpRhamBAD
sequencing_primer_3GTGCCGATATCTAAGCCTATTGAG
sequencing_primer_5gccacaattcagcaaattgtgaac
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namegRNA array
shRNA_sequence
size
species
100
Synthetic
tags
resistance markers :
3402
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA