This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CIB-VP64 (B1016)
catalog :
92037
citations: 4
Reference
Crespo E, Björefeldt A, Prakash M, Hochgeschwender U. Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo. J Vis Exp. 2021;: pubmed publisher
Hernández Candia C, Tucker C. Optogenetic Control of Gene Expression Using Cryptochrome 2 and a Light-Activated Degron. Methods Mol Biol. 2020;2173:151-158 pubmed publisher
Hernández Candia C, Wysoczynski C, Tucker C. Advances in optogenetic regulation of gene expression in mammalian cells using cryptochrome 2 (CRY2). Methods. 2019;: pubmed publisher
Pathak G, Spiltoir J, Höglund C, Polstein L, Heine Koskinen S, Gersbach C, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res. 2017;45:e167 pubmed publisher
product information
Catalog Number :
92037
Product Name :
CIB-VP64 (B1016)
article :
doi10.1093/nar/gkx260
id25589
pubmed_id28431041
bacterial resistance :
Kanamycin
cloning :
backbonepmCherryN1
backbone_mutationno mCherry tag.
backbone_originClontech
backbone_size3965
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsrGI
cloning_site_5SacI
promoterCMV
sequencing_primer_3custom
sequencing_primer_5custom
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1
geneCIB1
id829604
genbank_ids
mutation
nameCIB1-VP64
shRNA_sequence
size1224
species
3702
Arabidopsis thaliana
tags
location
tagFusion of plant CIB1 with VP64 activation domain. Intact NLS. No mCherry tag
plasmid copy :
VP64 was amplified from a plasmid from Charlie Gersbach (pcDNA-dCas9-VP64)
resistance markers :
852
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA