This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CRY-Gal DD (B1013)
catalog :
92035
citations: 4
Reference
Crespo E, Björefeldt A, Prakash M, Hochgeschwender U. Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo. J Vis Exp. 2021;: pubmed publisher
Hernández Candia C, Tucker C. Optogenetic Control of Gene Expression Using Cryptochrome 2 and a Light-Activated Degron. Methods Mol Biol. 2020;2173:151-158 pubmed publisher
Hernández Candia C, Wysoczynski C, Tucker C. Advances in optogenetic regulation of gene expression in mammalian cells using cryptochrome 2 (CRY2). Methods. 2019;: pubmed publisher
Pathak G, Spiltoir J, Höglund C, Polstein L, Heine Koskinen S, Gersbach C, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res. 2017;45:e167 pubmed publisher
product information
Catalog Number :
92035
Product Name :
CRY-Gal DD (B1013)
article :
doi10.1093/nar/gkx260
id25589
pubmed_id28431041
bacterial resistance :
Kanamycin
cloning :
backbonepmCherryC1
backbone_mutationIRES element between end of mCherry and SacI sites
backbone_originClontech
backbone_size5270
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Not1 between linker of CRY2 and Gal4 BD
cloning_site_5XhoI, SacI
promoter
sequencing_primer_3custom
sequencing_primer_5custom
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2
geneCRY2
id839529
genbank_ids
mutationFusion of plant CRY2 to Gal4 binding domain (AA1-65), downstream of mCherry-IRES element
nameCRY2
shRNA_sequence
size2103
species
3702
Arabidopsis thaliana
tags
resistance markers :
852
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA