This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CRY-tTA (B702)
catalog :
92032
citations: 1
Reference
Pathak G, Spiltoir J, Höglund C, Polstein L, Heine Koskinen S, Gersbach C, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res. 2017;45:e167 pubmed publisher
product information
Catalog Number :
92032
Product Name :
CRY-tTA (B702)
article :
doi10.1093/nar/gkx260
id25589
pubmed_id28431041
bacterial resistance :
Kanamycin
cloning :
backbonepmCherryN1
backbone_mutation
backbone_originClontech
backbone_size4994
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3custom
sequencing_primer_5custom
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2
geneCRY2
id839529
genbank_ids
mutationNLS on CRY2 is mutated
nameCRY2
shRNA_sequence
size1836
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagtTA2 (TetR fused to VP64)
plasmid copy :
Modified from plasmid in Tucker lab (already available on Addgene, CRY2(deltaNLS)-mCh) which contains mCherry fluorescent protein in vector mCherryN1 (Clontech). tTA2 domain was amplified from Addgene 36430.
resistance markers :
852
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA