CRY-GalVP16 (B695) | Addgene 92031 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

CRY-GalVP16 (B695)

catalog :

92031

citations: 3

Reference
Hernández Candia C, Tucker C. Optogenetic Control of Gene Expression Using Cryptochrome 2 and a Light-Activated Degron. Methods Mol Biol. 2020;2173:151-158 pubmed publisher
Hernández Candia C, Wysoczynski C, Tucker C. Advances in optogenetic regulation of gene expression in mammalian cells using cryptochrome 2 (CRY2). Methods. 2019;: pubmed publisher
Pathak G, Spiltoir J, Höglund C, Polstein L, Heine Koskinen S, Gersbach C, et al. Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2. Nucleic Acids Res. 2017;45:e167 pubmed publisher

product information

Catalog Number :

92031

Product Name :

CRY-GalVP16 (B695)

article :

doi	10.1093/nar/gkx260
id	25589
pubmed_id	28431041

bacterial resistance :

Kanamycin

cloning :

backbone

pmCherryC1

backbone_mutation

IRES element between mCherry and XhoI

backbone_origin

Clontech

backbone_size

5288

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XmaI
cloning_site_5	SacI
promoter
sequencing_primer_3	custom
sequencing_primer_5	custom
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2
gene	CRY2
id	839529

genbank_ids

mutation

name

CRY2

shRNA_sequence

size

species

3702

Arabidopsis thaliana