This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLJM1-FH-AGO2-5xE
catalog :
91981
citations: 1
Reference
Golden R, Chen B, Li T, Braun J, Manjunath H, Chen X, et al. An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017;542:197-202 pubmed publisher
product information
Catalog Number :
91981
Product Name :
pLJM1-FH-AGO2-5xE
article :
doi10.1038/nature21025
id25586
pubmed_id28114302
bacterial resistance :
Ampicillin
cloning :
backbonepLJM1
backbone_mutation
backbone_originDavid Sabatini Lab (Addgene #19319)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10
geneAGO2
id27161
genbank_ids
mutationS824E, S828E, T830E, S831E, S834E (5xE)
nameAGO2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG-HA
plasmid copy :
The parental AGO2 sequence used for this construct was amplified from a Thomas Tuschl lab plasmid (Addgene plasmid # 10822).
resistance markers :
328
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA