This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLJM1-FH-AGO2-WT
catalog :
91978
citations: 4
Reference
Wilson B, Su Z, Kumar P, Dutta A. XRN2 suppresses aberrant entry of tRNA trailers into argonaute in humans and Arabidopsis. PLoS Genet. 2023;19:e1010755 pubmed publisher
Su Z, Monshaugen I, Wilson B, Wang F, Klungland A, Ougland R, et al. TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer. Nat Commun. 2022;13:2165 pubmed publisher
Li H, Pinilla Macua I, Ouyang Y, Sadovsky E, Kajiwara K, Sorkin A, et al. Internalization of trophoblastic small extracellular vesicles and detection of their miRNA cargo in P-bodies. J Extracell Vesicles. 2020;9:1812261 pubmed publisher
Golden R, Chen B, Li T, Braun J, Manjunath H, Chen X, et al. An Argonaute phosphorylation cycle promotes microRNA-mediated silencing. Nature. 2017;542:197-202 pubmed publisher
product information
Catalog Number :
91978
Product Name :
pLJM1-FH-AGO2-WT
article :
doi10.1038/nature21025
id25586
pubmed_id28114302
bacterial resistance :
Ampicillin
cloning :
backbonepLJM1
backbone_mutation
backbone_originDavid Sabatini Lab (Addgene #19319)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expresses FLAG-HA-AGO2 (WT)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Unknown
cloning_site_5Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10
geneAGO2
id27161
genbank_ids
mutation
nameAGO2
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG-HA
plasmid copy :
The parental AGO2 sequence used for this construct was amplified from a Thomas Tuschl lab plasmid (Addgene plasmid # 10822).
resistance markers :
328
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA