This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP
catalog :
91877
citations: 1
Reference
Vo B, Li C, Morgan M, Theurillat I, Finkelstein D, Wright S, et al. Inactivation of Ezh2 Upregulates Gfi1 and Drives Aggressive Myc-Driven Group 3 Medulloblastoma. Cell Rep. 2017;18:2907-2917 pubmed publisher
product information
Catalog Number :
91877
Product Name :
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP
article :
doi10.1016/j.celrep.2017.02.073
id25564
pubmed_id28329683
bacterial resistance :
Ampicillin
cloning :
backbonepSpCas9(BB)-2A-GFP (PX458)
backbone_mutation
backbone_originFeng Zhang Lab (Addgene #48138)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
This vector also contains FLAG-tagged SpCas9-2A-EGFP.
growth strain :
Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterU6
sequencing_primer_3
sequencing_primer_5LKO.1 5'
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesGfi-1, Pal-1, Pal1
geneGfi1
id14581
genbank_ids
mutation
namegRNA targeting Gfi1
shRNA_sequenceGCTACGGCGACTTCGCGCCTG
size
species
10090
Mus musculus
tags
resistance markers :
1113
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA