This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)
catalog :
91819
citations: 1
Reference
Sdano M, Fulcher J, Palani S, Chandrasekharan M, Parnell T, Whitby F, et al. A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription. elife. 2017;6: pubmed publisher
product information
Catalog Number :
91819
Product Name :
CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)
article :
doi10.7554/eLife.28723
id25557
pubmed_id28826505
bacterial resistance :
Ampicillin
cloning :
backbonepRS315
backbone_mutationAddition of endogenous RPB1 promoter and 3 untranslated regions
backbone_originATCC
backbone_size7771
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
origin :
37
pi :
alt_names
RPB1, RPB220, SUA8, B220
cloning
clone_methodRestriction Enzyme
cloning_site_3Xho1
cloning_site_5Sph1
promoterEndogenous Rpb1 promoter
sequencing_primer_3M13R
sequencing_primer_5CTGTTTCCAACACCCAT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYDL140C, RPB1, RPB220, SIT1, SUA8
geneRPO21
id851415
genbank_ids
NM_001180200.1
mutation5xGly linker, PreScission Protease site (LEVLFQGP), and 10xHis tag inserted between residues P1455 and E1456; changed asparagine 1501 to lysine
nameRPO21
shRNA_sequence
size5268
species
4932
Saccharomyces cerevisiae
tags
location
tag10xHis
plasmid copy :
This plasmid was derived from pCK859, a gift from Craig Kaplan at Texas A&M University.
resistance markers :
2231
tags :
Low Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA